Journal: Data in Brief

Article Title: Dataset of RNA-seq profiles from THP-1-derived macrophages pretreated with AZD7648 and stimulated with CT DNA

doi: 10.1016/j.dib.2026.113124

Figure Lengend Snippet: Experimental design and sample IDs deposited in the NCBI database.

Article Snippet: For transcriptome profiling, cells were assigned to four experimental groups: untreated control (CON), AZD7648 (HY-111783, MedChemExpress) alone (AZD), CT DNA (HY-109517, MedChemExpress) alone (DNA), and AZD7648 pretreatment followed by CT DNA stimulation (AZD + DNA).

Techniques: Control

Journal: Data in Brief

Article Title: Dataset of RNA-seq profiles from THP-1-derived macrophages pretreated with AZD7648 and stimulated with CT DNA

doi: 10.1016/j.dib.2026.113124

Figure Lengend Snippet: Summary of clean sequencing data quality for all samples.

Article Snippet: For transcriptome profiling, cells were assigned to four experimental groups: untreated control (CON), AZD7648 (HY-111783, MedChemExpress) alone (AZD), CT DNA (HY-109517, MedChemExpress) alone (DNA), and AZD7648 pretreatment followed by CT DNA stimulation (AZD + DNA).

Techniques: Sequencing

Journal: Data in Brief

Article Title: Dataset of RNA-seq profiles from THP-1-derived macrophages pretreated with AZD7648 and stimulated with CT DNA

doi: 10.1016/j.dib.2026.113124

Figure Lengend Snippet: Summary of read alignment statistics for all samples.

Article Snippet: For transcriptome profiling, cells were assigned to four experimental groups: untreated control (CON), AZD7648 (HY-111783, MedChemExpress) alone (AZD), CT DNA (HY-109517, MedChemExpress) alone (DNA), and AZD7648 pretreatment followed by CT DNA stimulation (AZD + DNA).

Techniques:

Journal: Data in Brief

Article Title: Dataset of RNA-seq profiles from THP-1-derived macrophages pretreated with AZD7648 and stimulated with CT DNA

doi: 10.1016/j.dib.2026.113124

Figure Lengend Snippet: Sample correlation analysis and GSEA of the RNA-seq dataset.(A) Sample correlation heatmap of all RNA-seq samples. The color scale represents the pearson correlation coefficient between samples, ranging from blue (low correlation) to red (high correlation). (B) GSEA plot comparing the CON and DNA groups, showing significant enrichment of the cytosolic DNA‑sensing pathway.

Article Snippet: For transcriptome profiling, cells were assigned to four experimental groups: untreated control (CON), AZD7648 (HY-111783, MedChemExpress) alone (AZD), CT DNA (HY-109517, MedChemExpress) alone (DNA), and AZD7648 pretreatment followed by CT DNA stimulation (AZD + DNA).

Techniques: RNA Sequencing

Journal: Data in Brief

Article Title: Dataset of RNA-seq profiles from THP-1-derived macrophages pretreated with AZD7648 and stimulated with CT DNA

doi: 10.1016/j.dib.2026.113124

Figure Lengend Snippet: AZD7648 suppresses CT DNA-induced γH2AX accumulation. PMA-differentiated THP1 cells were pretreated with AZD7648 for 1 h and then transfected with CT DNA for 6 h, followed by Western blot analysis of γH2AX.

Article Snippet: For transcriptome profiling, cells were assigned to four experimental groups: untreated control (CON), AZD7648 (HY-111783, MedChemExpress) alone (AZD), CT DNA (HY-109517, MedChemExpress) alone (DNA), and AZD7648 pretreatment followed by CT DNA stimulation (AZD + DNA).

Techniques: Transfection, Western Blot